Production of L-lysine under submerged fermentation by Corynebacterium glutamicum using different plants' leaves


  • Theresa EZEDOM Delta State University, Abraka, Nigeria
  • Egoamaka Oliseneku Egbune
  • Solomon Adanoritsewo Atseponu
  • Mary Ogochukwu Charles
  • Blessed Achugbue Benson
  • Diana Ebbah
  • Promise Chika Amechi
  • Oghenetega Benjamin
  • Akpevweoghene Rejoice Egbodje
  • Lucky Ebinum
  • Blessing Ifechi Chukwudozie
  • Stephen Eboe
  • Ifeanyi Benedict Alexander
  • Sophia Fejiro Edijana
  • Nyerhovwo Tonukari


Agricultural leaves, submerged fermentation, Corynebacterium glutamicum, extract, boiling


Introduction: This study investigated the production of L-lysine using Corynebacterium glutamicum and
various leaf extracts (cassava, palm tree, maize, cowpea, cocoyam, and plantain). The study also explored
the activities of amylases and proteases, as well as the levels of total soluble proteins, reducing sugars,
glucose, flavonoid and phenolic contents, and pH changes.

Materials and Methods: Different treatments (extract, boiled extract, extract + C. glutamicum, boiled
extract + C. glutamicum) were examined for their effects on L-lysine concentration. Additionally, the
activities of amylases and proteases, as well as levels of total soluble proteins, reducing sugars, glucose,
flavonoid and phenolic contents, and pH changes, were analyzed.

Results: Maize leaf extract + C. glutamicum exhibited the highest L-lysine concentration (1.771a±0.1
mg/g), while boiled cassava leaf extract showed the lowest concentration (0.023b±0.1 mg/g). Palm tree
leaf extract had significantly higher reducing sugar levels compared to other extracts. Boiled plantain leaf
extract fermented by C. glutamicum had the highest total soluble protein level (9.5±0.2 mg/g), while
cassava leaf extract had the lowest (2.1±1.2 mg/g).

Conclusion: Submerged fermentation of leaf extracts using C. glutamicum can be utilized for L-lysine
production. The study highlights the influence of different leaf extracts and treatments on L-lysine
production, as well as on amylase and protease activities, total soluble protein levels, reducing sugars,
glucose, flavonoid and phenolic contents, and pH values. These findings provide valuable insights into
the potential application of this approach for lysine production.


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How to Cite

EZEDOM T, Egbune EO, Atseponu SA, Charles MO, Benson BA, Ebbah D, et al. Production of L-lysine under submerged fermentation by Corynebacterium glutamicum using different plants’ leaves. AJTMBR [Internet]. 2023 Jun. 5 [cited 2023 Oct. 1];6(1):12-24. Available from: