Assessment Of Haematological And Antioxidants Changes In Male Albino Wistar Rats Treated With Tramadol
Keywords:Tramadol, antioxidants, rat
Illicit drug use disorders are a major public health burden that contributes significantly to the global burden of disease and tramadol is one of the most common illicit psychoactive substances being abused especially amongst the young adults. This research aims to assess the haematological and antioxidants activities of male wistar rats treated with tramadol.
Materials and Methods
Thirty adult male Wistar rats weighing 120-180 g were selected for the study and was randomized into 6 groups. Group 1 was not treated within the period of the study before sacrificing, Group 2 to 5 received 30 mg/kg body weight of tramadol for 7, 14, 21 and 42 days respectively while treatment for group 6 was withdrawn for 3 weeks after 21 days treatment period before sacrificing. The animal's Brain, Liver, kidney and Testis were excised for biochemical analysis. Generated data were analyzed using SPSS package and results expressed as mean ± SEM.
Results obtained showed significant decrease in the haemtololgical parameters as well as in the WBC count, Catalase, SOD and Glutathione activities in the chronic tramadol-treated rats when compared to the normal control at p<0.05. This study also revealed that chronic tramadol use increases the level ofMDA significantly when compared with the non-treated group.
Tramadol consumption lowers RBC count, haemoglobin level, PCV, platelet count, WBC count, CAT, SOD, and GSH activities while significantly raising MDA levels. Therefore tramadol should only be used under medical supervision and only on prescription, avoiding indiscriminate and long-term.
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